| Name | cecropin |
| Synonyms | cecropin Anti-Cecropin Cecropin (antibacterial peptide) cecropin, Cecropin (antibacterial peptide) cecropin, Cecropin (antibacterial peptide) |
| CAS | 80802-79-5 |
| Physical and Chemical Properties | Chemical Properties The N-terminal 15 amino acids in the structure of this product are mostly hydrophilic and positively charged. The C- terminal is mostly hydrophobic amino acids. Bactericidal peptides are stable to heat, still active in water bath at 100 ℃ for half an hour, not easy to be hydrolyzed by protease, stable to nuclease, amylase, disulfide reagent, and isoelectric point 9.8-10.7. |
| Use | Use of bacteria, Virus, the original organisms and some cancer cells have a killing effect. More sensitive than penicillin, erythromycin, is a potential new antibiotic drugs, can be taken orally. |
| Raw Materials | Ribonucleic acid Lysozyme |
silkworm chrysalis immunized by injecting silkworm chrysalis with inactivated Escherichia coli K12D31 strain. Puncture immunized silkworm pupae, centrifugation, collection of blood lymphocytes.
Silkworm chrysalis [Escherichia coli] & rarr; Immune silkworm chrysalis [puncture, centrifugation] & rarr; Blood lymphocyte collection solution
Sephadex G-75 column chromatography with acetic acid to adjust the blood lymphocyte collection solution, pH 4, heating to 60 ℃, keeping for 10-30min, quick cooling. Centrifuge at 20000 ×g, take the supernatant, adjust the pH to neutral with sodium carbonate, and use Sephadex G-75 column chromatography. The pH 6.2 phosphate buffer (0.2 mol/L) was eluted and detected by nucleic acid protein detector. The bactericidal peptide (crude) and lysozyme were collected respectively.
Chromatography, dialysis, freeze-drying, use CM-Sephadex CL-6B chromatography to elute the crude germicidal peptide eluate with pH 6.2 phosphate buffer (0.2 mol/L), and then use Phenyl-Sephadex-4B hydrophobic chromatography for the elution part. The germicidal peptide is adsorbed in the column, and is eluted with 0.1-0.5 mol/L phosphate buffer in reverse gradient to collect the active part. Dialysis to remove salt, freeze-dried to obtain bactericidal peptide.
Bactericidal peptide eluent [CM-Sephadex CL-6B]& rarr; Eluate [Phenyl-Sephadex-4B]& rarr; Eluate [dialysis] & rarr; Dialysate [freeze drying] & rarr; Sterile peptide freeze-dried products.
| chemical properties | the n-terminal 15 amino acids in the structure of this product are mostly hydrophilic and positively charged. The C end is mostly hydrophobic amino acids. Bactericidal peptide is stable to heat, it is still active after heating in a water bath at 100 ℃ for half an hour, is not easy to be hydrolyzed by protease, is stable to nuclease, amylase, disulfide reagent, and has an isoelectric point of 9.8-10.7. |
| use | has a killing effect on bacteria, viruses, organisms and certain cancer cells. It is more sensitive than penicillin and erythromycin. It is a potential new antibiotic drug and can be taken orally. |
| production method | silkworm pupa immunization silkworm pupa was injected with inactivated Escherichia coli K12D31 strain to immunize silkworm pupa. Puncture immunized silkworm pupa, centrifugation, blood lymphocytes were collected. Silkworm chrysalis [Escherichia coli] → immune silkworm chrysalis [puncture, centrifugation] → blood lymphocyte collection liquid Sephadex G-75 column chromatography with acetic acid to adjust blood lymphocyte collection liquid, pH 4, heating to 60 ℃, keeping for 10-30min, quick cooling. Centrifuge at 20000 ×g, take the supernatant, adjust the pH to neutral with sodium carbonate, and use Sephadex G-75 column chromatography. The pH 6.2 phosphate buffer (0.2 mol/L) was eluted and detected by nucleic acid protein detector. Bactericidal peptide (crude) and lysozyme were collected respectively. Chromatography, dialysis, freeze-drying, use CM-Sephadex CL-6B chromatography to elute the crude germicidal peptide eluate with pH 6.2 phosphate buffer (0.2 mol/L), and then use Phenyl-Sephadex-4B hydrophobic chromatography for the elution part. The germicidal peptide is adsorbed in the column, and is eluted with 0.1-0.5 mol/L phosphate buffer in reverse gradient to collect the active part. Dialysis desalting, freeze-drying to obtain bactericidal peptide. Bactericidal peptide eluent [CM-Sephadex CL-6B]→ eluent [Phenyl-Sephadex-4B]→ eluent [dialysis] → dialysate [freeze-drying] → bactericidal peptide freeze-dried product. |